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Download PDF by Mohler F.L.: A Photo-Ionization Experiment with Hydrogen

By Mohler F.L.

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Review: aptamers in microfluidic chips. Anal Chim Acta 2010;683:12e20. [129] Zhao Q, Li X-F, Shao Y, Le XC. Aptamer-based affinity chromatographic assays for thrombin. Anal Chem 2008;80:7586e93. [130] Zhao Q, Li X-F, Le XC. Aptamer-modified monolithic capillary chromatography for protein separation and detection. Anal Chem 2008;80:3915e20. [131] Mairal T, Oezalp VC, Sanchez PL, Mir M, Katakis I, O’Sullivan CK. Aptamers: molecular tools for analytical applications. Anal Bioanal Chem 2008;390:989e1007.

54] Clonis YD, Labrou NE, Kotsira V, Mazitsos K, Melissis S, Gogolas G. Biomimetic dyes as affinity chromatography tools in enzyme purification. J Chromatogr A 2000;891:33e44. 20 1. AFFINITY CHROMATOGRAPHY [55] Denizli A, ikin E. Dye-ligand affinity systems. J Biochem Biophys Meth 2001;49:391e416. [56] Labrou NE. Dye-ligand affinity chromatography for protein separation and purification. In: Walker JM, editor. Methods in molecular biology, affinity chromatography: methods and protocols. Totowa, NJ: Humana Press; 2000.

Since derivatizing reactions can be performed either precolumn, and affect the separation of the compounds, or postcolumn, and affect only the detection step, there is some flexibility in the selected approach. Occasionally, the derivatization reaction is performed close to the start of an isolation procedure to increase the recovery of the analytes, to ensure their stability to the conditions employed in the isolation procedure, or to increase the selectivity of the method for the target compounds.

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A Photo-Ionization Experiment with Hydrogen by Mohler F.L.

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